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Home collection of urine specimens--boric acid bottles or Dipslides?

机译:尿液标本的家庭采集-硼酸瓶还是Dipslides?

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摘要

Sterile mid stream specimens of urine (MSSU) were obtained from 84 children in a hospital outpatient department. All 84 children collected urine at home by one of two Dipslide methods and by collection into boric acid within 24 hours of the hospital collected MSSU. The samples collected at home were posted to the hospital. Thirty six of the Dipslides (43%) and nine of the boric acid samples (10%) were not sterile but none had a pure growth of a single organism of greater than 10(5) organisms/ml. In addition, 17 of the Dipslides (20%) were returned with one or both media detached and therefore could not be relied upon to exclude urinary tract infection. In a second part to the study, 95 urines which showed a significant growth in primary culture were also cultured after storage in boric acid. Inhibition was noted in nine samples after storage in boric acid, seven of which were in underfilled bottles. Transport of specimens in boric acid produced less contamination than Dipslides but may inhibit growth in a small number of specimens. Technical failures with Dipslides were disappointingly high.
机译:从医院门诊的84名儿童中获得了无菌的中游尿液标本(MSSU)。所有84名儿童均采用两种Dipslide方法之一在家中收集尿液,并在医院收集MSSU后24小时内收集到硼酸中。在家收集的样品被送到医院。 Dipslides中有36种(43%)和硼酸样品中有9种(10%)不是无菌的,但是没有一个微生物的纯生长大于10(5)个生物/ ml。此外,退回的17种Dipslides(占20%)的一种或两种介质均已分离,因此不能依靠它排除尿路感染。在研究的第二部分中,在硼酸中储存后,还培养了95条在原代培养中显示出显着增长的尿液。在硼酸中保存后的9个样品中发现有抑制作用,其中7个在底部填充的瓶子中。样品在硼酸中的迁移所产生的污染要少于Dipslides,但可能会抑制少量样品的生长。 Dipslides的技术失败率很高,令人失望。

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